Brisbane Broncos coach Wayne Bennett confirmed on Friday that Greg Eden would start on the wing in place of the injured Corey Oates.
It ended days of speculation surrounding the rumoured early return of winger Lachlan Maranta, who himself is recovering from an off-season wrist injury.
Saturday night's match against Penrith will now be of utmost importance for Eden as he looks to cement his position in the Broncos' starting 17.
Bennett said Eden needs to take his opportunities after a shaky debut against the Eels two weeks ago.
"It's a chance for him to step up. He needs to now take his opportunity," Bennett said.
"He was a bit patchy when we played Parramatta but he's better than that.
"I don't expect him to have any more work coming his way than normal. He's quite good under the high ball. I've got no control over the tactics Penrith use.
"Greg's done all the training so I'm very confident in him."
Brisbane will start 1-17 and Eden must now prove he is first grade material after making four errors in a below-par performance in Round 1.
Maranta trained hard all week and the 23-year-old is expected to be fit to return within the next fortnight.
With Oates out for at least six weeks, the pressure is on Brisbane's English import to perform well this weekend and keep Maranta out of the side.
Maranta has a strong case for selection when he returns as he finished as Brisbane's leading try-scorer in 2015 with 15 tries in 20 games.
Bennett says although Maranta won't be back this week, his return is not far away.
"Lachlan Maranta is pretty close to returning but he went to the doctor the other day and the doctor wasn't quite happy with where he's at," he said.
"He's not far off. He will probably be another two weeks.
"I didn’t think he would be back this week. He needs to be cleared by the doctor before I have any thoughts of bringing him into the side. The doctor wasn't totally happy with where his wrist is at so he won't be back this week."